Journal: Cancer Medicine

Article Title: SNGH16 regulates cell autophagy to promote Sorafenib Resistance through suppressing miR‐23b‐3p via sponging EGR1 in hepatocellular carcinoma

doi: 10.1002/cam4.3020

Figure Lengend Snippet: The lncRNA microarray data from Hep3B and Hep3B/So cells

Article Snippet: Human LncRNA Microaray V1.0 (Arraystar) was used to analyze the data of five replicate samples, calculate the p ‐value of the gene expression difference and statistical significance, and use the Fold Change (FC) ≥1.5 or ≤−1.5, P < .05 for t test as a criterion for differential gene screening.

Techniques: Microarray

Journal: Nature Communications

Article Title: circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity

doi: 10.1038/s41467-020-20527-z

Figure Lengend Snippet: a A heatmap and volcano plot show 109 differentially expressed circRNAs in three paired samples of NSCLC by Arraystar Human Circular RNA Microarray. Cut off is Log 2 (fold change) > 1 or <−1.0, P value < 0.05. b Analysis for RNA levels of circNDUFB2 in additional 52 paired samples of NSCLC (left). Expression proportions of circNDUFB2 in histogram and pie chart (right). Log 2 (T/N expression) value > 1 as significantly higher expression, which <−1 as significantly lower expression, and between −1 and 1 as no significant change. N nontumorous tissue, T tumorous tissue. Data are presented as mean ± s.d. P values are calculated by paired two-sided t -test. n = 52 biologically independent paired tissues of NSCLC. c The backsplice junction site of circNDUFB2 was identified by Sanger sequencing. d PCR analysis for circNDUFB2 and β-actin in cDNA and genomic DNA. RH random hexamers, OdT oligo(dT) 18 primers, gDNA genomic DNA. Two independent experiments were carried out with similar results. e Analysis for RNA levels of circNDUFB2 and NDUFB2 after treatment with RNase R. n = 3 biologically independent samples. f Analysis for RNA abundance of circNDUFB2 and NDUFB2 treated with Actinomycin D (2 μg/ml) at the indicated time point. n = 3 biologically independent samples. g RNA levels of circNDUFB2 , NDUFB2 , β-actin , and U1 in the nuclear and cytoplasmic fractions of A549 and H1650 cells. n = 3 biologically independent samples. h RNA FISH analysis for circNDUFB2 in A549 cells, scale bar = 15 μm. Two independent experiments were carried out with similar results. Data are presented as mean ± s.d in e – g . P values are calculated by unpaired two-sided t -test in a , e – g .

Article Snippet: The labeled cRNAs were hybridized onto the Arraystar Human Circular RNA Microarray V1.0 (6x7K, Arraystar), which contains 5396 probes specific for human circular RNAs backsplice junction region.

Techniques: Microarray, Expressing, Sequencing

Journal: BMC Complementary Medicine and Therapies

Article Title: A circRNA-miRNA-mRNA network plays a role in the protective effect of diosgenin on alveolar bone loss in ovariectomized rats

doi: 10.1186/s12906-020-03009-z

Figure Lengend Snippet: Differentially expressed exonic circRNAs of alveolar bone of rats in the DIO in comparison to those in the OVX group ( p <0.05)

Article Snippet: Thereafter, the labeled cRNA was subjected to hybridization, washing and scanning on Arraystar mouse circRNA Array v1.0 using DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA).

Techniques: Comparison

Journal: BMC Complementary Medicine and Therapies

Article Title: A circRNA-miRNA-mRNA network plays a role in the protective effect of diosgenin on alveolar bone loss in ovariectomized rats

doi: 10.1186/s12906-020-03009-z

Figure Lengend Snippet: RT-qPCR validation of 10 differentially expressed circRNAs. Effect of DIO on the gene expression of rno_circRNA_000034, rno_circRNA_016717, rno_circRNA_005275, rno_circRNA_007431, rno_circRNA_003113, rno_circRNA_014839, rno_circRNA_002656, rno_circRNA_002387, rno_circRNA_002043 and rno_circRNA_001963. # p < 0.05 vs . the OVX group

Article Snippet: Thereafter, the labeled cRNA was subjected to hybridization, washing and scanning on Arraystar mouse circRNA Array v1.0 using DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA).

Techniques: Quantitative RT-PCR, Biomarker Discovery, Gene Expression

Journal: BMC Complementary Medicine and Therapies

Article Title: A circRNA-miRNA-mRNA network plays a role in the protective effect of diosgenin on alveolar bone loss in ovariectomized rats

doi: 10.1186/s12906-020-03009-z

Figure Lengend Snippet: Primers of differentially expressed exonic circRNAs

Article Snippet: Thereafter, the labeled cRNA was subjected to hybridization, washing and scanning on Arraystar mouse circRNA Array v1.0 using DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA).

Techniques:

Journal: BMC Complementary Medicine and Therapies

Article Title: A circRNA-miRNA-mRNA network plays a role in the protective effect of diosgenin on alveolar bone loss in ovariectomized rats

doi: 10.1186/s12906-020-03009-z

Figure Lengend Snippet: A gene axis of circRNA, miRNA and mRNA extracted from the circRNA-miRNA-mRNA network associated with the roles exerted by DIO on alveolar bone loss of rats subjected to OVX. The downregulated genes are presented in green, while the upregulated genes are presented in red

Article Snippet: Thereafter, the labeled cRNA was subjected to hybridization, washing and scanning on Arraystar mouse circRNA Array v1.0 using DNA Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA).

Techniques: